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This is a substrate for Immunofluorescence, enabling a highly sensitive detection of cellular surface antigen expression. A highly sensitive CLAMP method (quinone methide-based catalyzed signal amplification) can be applied to live/fixed cells or tissue sections. In this method, using ?-galactosidase-labeled secondary antibody and newly developed fluorescent dye CLAMP F405, the cells expressing a specific cell surface protein are selectively and highly sensitively stained.