...Tube 1 Sodium Citrate containing separation tube Tube 2 Calcium Chloride containing activation tube 10 ml of blood is drawn into Tube 1 under vacuum 1. Centrifuge at 1900 RCF for 8 minutes A portion of RBC along with all plasma is collected and transferred to Tube 2 and gently mixed. Tube 2 is centrifuged again at 2000 RCF for 4 minutes All activated plasma is collected and applied Since...

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